Dec 28, 2020 Western blot was performed as previously descri- bed22. The blots were incubated with antibodies against cleaved-caspase-3 (ab13847, Abcam, 


Rabbit monoclonal antibody (EPR165) against Cdk1 and rabbit polyclonal antibody against Caspase 3 (#ab13847) were used at a 1:200 dilution for immunohistochemistry (IHC) with an HRP-labeled anti-rabbit IgG (#G-21231) (1:500 for IHC) secondary antibody purchased from Invitrogen.

The Active Caspase 3 antibody was conjugated to HRP complex using an Abcam HRP Conjugation Kit (ab102890), diluted to approximately 3µg/mL, applied to tissues for 15 minutes, and detected with Abcam Steady DAB/Plus (ab103723) for 5 minutes. active Caspase 3 P Flow Cyt, ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, Rb Hu, Ms, Rt, Dm, InMtj, ab13847 IHC (PFA fixed), WB RMk, Xl, Zfsh active Caspase 3 M WB Rb Hu ab77973 and rabbit anti-caspase-3 (cat. no. ab13847; 1:1,000; Abcam). The blots were then washed with TBST and incubated with the secondary antibody (cat. no.-2306; cat. ZB no.


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Sc22948. IF. Anti-CD3 (. Alexa Fluor®. 647-conjugated). BioLegend. ogy, #2772), pro-caspase3 (1:1000, Abcam, #ab13847), cleaved caspase3 (1: 1000, Abcam, #ab49822), cyt-c.

Age-resistant paper. We plant two trees for   Jun 28, 2018 The embryos were then incubated in the blocking solution with a rabbit anti- Caspase-3 primary antibody (1:500, ab13847) for 72 h at 4°C.

Chronic cerebral hypoperfusion (CCH) is a main cause of vascular dementia and is also an etiological factor of neurological diseases and mental disorders. However, few treatments are available for CCH, and new medications are needed. In the present study, we employed a rat model of CCH that was based on bilateral common carotid artery occlusion and investigated the therapeutic effects of mouse and rabbit specific hrp dab detection ihc kit/product/Abcam. Average 97 stars, based on 3 article reviews.


Breast cancer is one of the most common malignant tumor in women worldwide with a high morbidity and mortality. It is estimated that there are 2 million new cases and 630,000 deaths from breast cancer every year, accounting for 14% of all cancer-related deaths among women worldwide (Ferlay et al., 2019). Rabbit polyclonal antibody against Caspase 3 (#ab13847), rabbit monoclonal antibody (EPR17060) against Cyclin B1 (#ab181593), rabbit monoclonal antibody (EPR18107) against Caspase 9 (#ab202068), mouse monoclonal antibody (7F7AB10) against AIF (#ab110327), and rabbit polyclonal antibody against cytochrome (#ab90529) were used at a 1:500 dilution 2020-09-01 · 2.6. Western blot assay. The treated H9c2 cells were harvested, and the treated myocardial tissues of rats were collected and ground on the ice.


The blots were incubated with antibodies against cleaved-caspase-3 (ab13847, Abcam,  anti-Map1lc3a/b (1:3,000, NB100–2331) was purchased from Novus Biologicals, anti-active Casp3/caspase 3 antibody (1:500, ab13847) was purchased from  CD95 Antibody. Annexin V-FITC Apoptosis Detection Kit. Annexin V-Cy3 Apoptosis Detection Kit ab14144. Cat.No ab2302 ab16048 ab13847 ab2996 ab19139. Feb 10, 2021 (PCNA, AB13847, Abcam, MA) or an IgG that recognizes cleaved caspase-3 (1: 250, AB13847, Abcam, MA), respectively. Rat anti- mouse CD3  ThermoFisher. Scientific. Rabbit anti-mouse Ki-67 ab15580.
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Consequently, attenuating inflammation by reducing local macrophage accumulation is an appealing approach. This can potentially be accomplished by either blocking blood monocyte recruitment to the plaque or increasing macrophage apoptosis and emigration.

Active caspase-3, which is a proapoptotic factor, has been shown to reduce cardiac contractility, causing cardiac dysfunction in many pathological diseases.
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The Ab13847) Anti-Caspase-3 Antibody Zebrafish reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact zebrafish Antibody. Other Ab13847) products are available in stock.

If you have written or know of … I am pleased to let you know that ab2302, ab13847, ab44976 and ab4051 have been tested on rat samples and in IHC-P and would be covered by our guarantee for use in this species and application. I can confirm that ab13847 tested in IHC-P on adult rat brain in an Abreview. I can recommend to review this on the datasheet for further information. ab13847 recognizes a cleaved form of Caspase 3 (~17 kDa) after apoptosis has been induced in wildtype cells and not Caspase 3 knockout cells. Some customers have used this antibody successfully in IHC-P however our latest tests were unsuccessful and therefore we can no longer guarantee this application. active Caspase 3 P Flow Cyt, ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, Rb Hu, Ms, Rt, Dm, InMtj, ab13847 IHC (PFA fixed), WB RMk, Xl, Zfsh active Caspase 3 M WB Rb Hu ab77973 Hong X, O Donnell J, Salazar C, Van Brocklyn J, Barnett K, Pearl D, et al.The selective Aurora-A kinase inhibitor MLN8237 (alisertib) potently inhibits proliferation of glioblastoma neurosphere tumor stem-like cells and potentiates the effects of temozolomide and ionizing radiation.